Changes in the Enamel Protein during Enamel Formation

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[Changes in the enamel protein during enamel formation].

要 旨:幼 若エナ メル質 中に含 まれ ている有機質 のほ とん どを占めている蛋白質 は多成分系 であ る事 が知 ら れ てい る。それ らの うちの どの成分が生理的 に重要 なのかを明 らかにする 目的で,先 ずラ ッ ト幼若 エナ メル 蛋 白質についてゲル濾過 クロマ トグラフィー とSDS-ポ リアク リル ア ミ ドゲル電気泳動 を行 な って,石 灰 化 の進行 に伴 う蛋 白質 のパ ターンの変化 を調 べた。続 いて,ラ ッ トに3H-prolineを 静注 し,そ のエナメル質 へ の取込み の時間的 な変化 をゲル濾過 ク ロマ トグラフィー とポ リアク リル ア ミ ドゲル電気泳動 を行な って調 べ た。 その結果 は若 いstageのmain componentで ある分子量約20,000のproline,glutamic acid,histidi...

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Endocytosis and Enamel Formation

Enamel formation requires consecutive stages of development to achieve its characteristic extreme mineral hardness. Mineralization depends on the initial presence then removal of degraded enamel proteins from the matrix via endocytosis. The ameloblast membrane resides at the interface between matrix and cell. Enamel formation is controlled by ameloblasts that produce enamel in stages to build t...

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Proteomic Evaluation of Acquired Enamel Pellicle during In Vivo Formation

Acquired enamel pellicle (AEP) is a protein film that forms on the enamel surface of teeth by selective adsorption of proteins and peptides present in the mouth. This protein film forms the interface between enamel and the damage oral biofilm, which modulates the attachment of bacteria found in oral biofilm. The overall goal of this study was to gain insight into the biological formation of the...

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Amelogenin-cytokeratin 14 interaction in ameloblasts during enamel formation.

The enamel protein amelogenin binds to the GlcNAc-mimicking peptide (GMp) (Ravindranath, R. M. H., Tam, W., Nguyen, P., and Fincham, A. G. (2000) J. Biol. Chem. 275, 39654-39661). The GMp motif is found in the N-terminal region of CK14, a differentiation marker for ameloblasts. The binding affinity of CK14 and amelogenin was confirmed by dosimetric binding of CK14 to recombinant amelogenin (rM1...

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Protein-mediated enamel mineralization.

Enamel is a hard nanocomposite bioceramic with significant resilience that protects the mammalian tooth from external physical and chemical damages. The remarkable mechanical properties of enamel are associated with its hierarchical structural organization and its thorough connection with underlying dentin. This dynamic mineralizing system offers scientists a wealth of information that allows t...

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ژورنال

عنوان ژورنال: THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN

سال: 1973

ISSN: 1884-5185,0300-9149

DOI: 10.5357/koubyou.40.25